|
KMID : 1025520130550050417
|
|
Journal of Animal Science and Technology
2013 Volume.55 No. 5 p.417 ~ p.425
|
|
|
Comparison of Vitrification and Slow Freezing for the Cryopreservation of Chicken Primordial Germ Cell (Ogye)
|
|
Kim Sung-Woo
Ko Yeoung-Gyu
Byun Mi-Jeong
Do Yoon-Jung
Han Jae-Yong
Kim Dong-Hoon
Seong Hwan-Hoo
Kim Hyun
|
|
|
Abstract
|
|
|
|
We sought to provide a method for freezing and preserving primordial germ cells, or an avian germ cell of a bird, as a material for developmental engineering or species preservation. The aim of this study was to compare the efficacy of slow freezing with a vitrification method for the cryopreservation of chicken primordial germ cells(PGCs). PGCs obtained from the germinal gonad of day 5.5-6 day(stage 28) cultured chick embryos, using the MACS method, were classified into two groups: slow freezing and vitrification. We examined the viability of PGCs after Cryopreservation. Four freezing methods were compared with each other, including the following: Method 1: The PGCs were frozen by a programmed freezer in a plastic straw, including 2.0M ethylene glycol(EG) as cryoprotective additive(slow freezing) Method 2: The PGCs were vitrified in a plastic straw, including 8.0 M EG, plus 7% polyvinylpyrrolidone(PVP)(rapid freezing). Method 3: The slow freezing was induced with a cryotube including 2.0 M EG Method 4: The PGCs were frozen in a cryotube including 10% dimethyl suloxide(DMSO)(rapid freezing). After freezing and thawing, survival rates of the frozen-thawed PGCs from Method 1 to 4were 76.4%, 70.6%, 80.5% and 78.1%(p<0.05), respectively. The slow freezing(－80℃ programmed freezer) method may provide better survival rates of frozen-thawed PGCs than the vitrification method for the cryopreservation of PGCs. Therefore, these systems may contribute to the cryopreservation of a rare avian species.
|
|
|
KEYWORD
|
|
|
Primordial germ cells (PGCs), EG, Viability, Korean native chicken (Ogye), Slow freezing
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|